林睿博士

清华大学生物医学交叉研究院助理教授，北京生命科学研究所研究员

Rui Lin, Ph.D. Assistant Professor, TIMBR, Assistant Investigator, NIBS, Beijing, China

教育经历 Education

2018 博士，北京大学、清华大学和北京生命科学研究所联合培养博士研究生项目

PhD, Peking University-Tsinghua University-National Institute Biological Sciences (PTN) Joint Graduate Program

2012 学士, 厦门大学

B.S.,Xiamen University, China

工作经历 Professional Experience

2022.6- 清华大学生物医学交叉研究院，助理教授，北京生命科学研究所, 研究员。

2022.6- Assistant Professor, TIMBR, Assistant Investigator,National Institute Biological Sciences, Beijing

2018.8-2022.5 博士后，北京生命科学研究所

Postdoctoral Research Fellow,National Institute Biological Sciences, Beijing

研究概述 Research Description

小胶质细胞是中枢神经系统中最主要的常驻免疫细胞。作为一种特殊的巨噬细胞，小胶质细胞广泛分布在中枢神经系统中。处于静息状态的小胶质细胞持续监测周围环境，当遭遇如细胞损伤、炎症相关分子等刺激时，小胶质细胞进入多种激活状态，并通过吞噬、迁移、细胞因子释放等多种方式进行响应和修复。在发挥免疫功能之外，小胶质细胞也广泛参与神经环路的调控，对神经环路发育以及突触可塑性等都至关重要。小胶质细胞功能异常是引起神经系统衰老和神经系统疾病的重要因素。例如，近期人类遗传学研究发现，绝大多数神经退行性疾病的易感基因都在小胶质细胞中特异性表达。因此，小胶质细胞是治疗中枢系统疾病的重要靶点。我们实验室通过开发针对小胶质细胞的新技术，全面研究在正常生理状态和疾病模型中小胶质细胞增殖、死亡和细胞密度维持的动态调节机制，并在此基础之上探索基于小胶质细胞的基因和细胞疗法。同时，我们也致力于其他神经科学领域的新技术开发。

As the resident macrophages, microglia account for about 10% of the total cell population in the central nervous system (CNS). Microglia conduct active surveillance, and initiate rapid innate and adaptive immune responses upon encountering immune assaults. Beyond their functions in immunity, microglia have multifaceted roles in controlling neural circuit development and plasticity. Microglial dysfunction is a key factor in CNS ageing and in the progression of CNS diseases including neurodegenerative disorders and brain cancers. Clinical studies have identified risk-associated alterations in genes that are highly expressed in microglia, highlighting the engagement of microglia in CNS disease progression and the potential for targeting microglia for therapeutic interventions. By developing new molecular and genomic tools, our lab studies the microglia population dynamics under normal physiological conditions and in disease models. We are also interested in developing microglia-based therapies and neurotechnologies for broad neuroscience fields.

发表文章 Publications

(# corresponding author, * co-first author)

Key research papers:

1.Lin, R.*#, Zhou, Y.*, Yan, T.*, Wang, R.*, Li, H., Wu, Z., Zhang, X, Zhou, X, F., Zhang, L., Li, Y., Luo, M.#, (2022). Directed evolution of adeno-associated virus for efficient gene delivery to microglia.Nature Methods, in press.

2.Lin, R.*#, Liang, J.*, Wang, R., Yan, T., Guo, Z., Liu, Y., Feng, Q., Sun, F., Li, Y., Li, A., Gong, H., Luo, M.#, (2020). The raphe dopamine system controls the expression of incentive memory.Neuron106 (3), 498-514.

3.Lin, R.*, Wang, R.*, Yuan, J.*, Feng, Q., Zhou, Y., Zeng, S., Ren, M., Jiang, S., Ni, H., Zhou, C., Gong, H., Luo, M., (2018). Cell-type-specific and projection-specific brain-wide reconstruction of single neurons.Nature Methods15, 1033–1036.

4.Lin, R., Feng, Q., Li, P., Zhou, P., Wang, R., Liu, Z., Wang, Z., Qi, X., Tang, N., Shao, F., Luo, M., (2018). A hybridization-chain-reaction-based method for amplifying immunosignals.Nature Methods15, 275–278.

Review papers:

1.Lin, R.*#, Liang, J.*, Luo, M.#, (2021). The raphe dopamine system: roles in salience encoding, memory expression, and addiction.Trends in Neurosciences44, 366–377.

2. Liu, Z.,Lin, R., Luo, M., (2020). Reward contributions to serotonergic functions.Annual Review of Neuroscience. 43, 141–162.

3.Lin, R., Yan, T., Luo, M., (2019). The two faces of PVN CRF neurons.Nature Neuroscience22, 508–510.

4.Lin, R.#, Yi, L., Luo, M., (2018). A neural circuit driving maternal behaviors.Neuron98 (1), 6-8.

Other research papers:

1. Feng, Q., An, S., Wang, R.,Lin, R., Li, A., Gong, H., Luo, M., (2021). Whole-brain reconstruction of neurons in the ventral pallidum reveals diverse projection patterns.Frontiers in Neuroanatomy15, 108.

2. Lu, L., Ren, Y., Yu, T., Liu, Z., Wang, S., Tan, L., Zeng, J., Feng, Q.,Lin, R., Liu, Y., Guo, Q., Luo, M., (2020). Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus.Nat Commun11, 262.

3. Ren, J.*, Isakova, A.*, Friedmann, D.*, Zeng, J.*, Grutzner, S., Pun, A., Zhao, G.Q., Kolluru, S.S., Wang, R.,Lin, R., Li, P., Li, A., Raymond, J.L., Luo, Q., Luo, M., Quake, S.R., Luo, L., (2019). Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei.eLife8, e49424.

4. Zhao, Z., Wang, L., Gao, W., Hu, F., Zhang, J., Ren, Y.,Lin, R., Feng, Q., Cheng, M., Ju, D., Chi, Q., Wang, D., Song, S., Luo, M., Zhan, C., (2017). A central catecholaminergic circuit controls blood glucose levels during stress.Neuron95 (1), 138-152.

5. Zhang, J., Tan, L., Ren, Y., Liang, J.,Lin, R., Feng, Q., Zhou, J., Hu, F., Ren, J., Wei, C., Yu, T., Zhuang, Y., Bettler, B., Wang, F., Luo, M., (2016). Presynaptic excitation via GABA_Breceptors in habenula cholinergic neurons regulates fear memory expression.Cell166 (3), 716-728.

6. Ren, J., Zhang, M.-J., Li, T.-M., Zhang, J.,Lin, R., Chen, S., Luo, M., and Dong, M.-Q. (2016). Quantitative proteomics of sleep-deprived mouse brains reveals global changes in mitochondrial proteins.PLOS ONE11(9): e0163500.

7. Wang, D., He, X., Zhao, Z., Feng, Q.,Lin, R., Sun, Y., Ding, T., Xu, F., Luo, M., Zhan, C., (2015). Whole-brain mapping of the direct inputs and axonal projections of POMC and AgRP neurons.Frontiers in Neuroanatomy9, 40.

8. Guo, Q., Zhou, J., Feng, Q.,Lin, R., Gong, H., Luo, Q., Zeng, S., Luo, M., Fu, L., (2015). Multi-channel fiber photometry for population neuronal activity recording.Biomedical Optics Express6 (10), 3919-393.

9. Guo, Q., Wang, D., He, X., Feng, Q.,Lin, R., Xu, F., Fu, L., Luo, M., (2015). Whole-brain mapping of inputs to projection neurons and cholinergic interneurons in the dorsal striatum.PLOS ONE10(4): e0123381.