Feng Shao, Ph.D.

Member of Chinese Academy of Sciences, Professor, TIMBR, Investigator and Deputy Director for Academic Affairs, NIBS, Beijing, China

1996    B.S. Applied Chemistry, Peking University, Beijing, China

1999    M.S. Molecular Biology, Institute of Biophysics, ChineseAcademy of Sciences, Beijing, China

2003    Ph.D. Biological Chemistry, University of Michigan MedicalSchool,Ann Arbor,  MI, USA

2012-present    Member of Chinese Academy of Sciences, Professor, TIMBR, Investigator and Deputy Director for Academic Affairs, NIBS, Beijing, China

2009-2012    Associate Investigator, National Institute of Biological Sciences, Beijing, China

2005-2009    Assistant Investigator, National Institute of Biological Sciences, Beijing, China

2004-2005    Damon Runyon Postdoctoral Research Fellow, Harvard Medical School, Boston, USA

2003-2004    Postdoctoral Research Fellow, School of Medicine, University of California, San Diego, USA

Dr. Feng Shao’s laboratory is interested in studying molecular mechanisms of bacterial infection and host innate immunity defense. Bacterial pathogens use specialized secretion systems such as type III/IV secretion system to inject effector proteins into host cells, serving as a key and universal virulence mechanism. The effectors usually harbor a unique and potent activity that modulates the function of key signaling molecules in the host, and this plays a critical role in bacterial survival and systemic infections. Using pathogens such asShigella,Salmonella,Enteropathogenic E. coli(EPEC),LegionellaandBurkholderiaas the model, we are working to discover and reveal some novel and common biochemical mechanisms utilized by bacterial effectors in modulating host signal transduction pathways. Our recent work has led to several interesting discoveries. 1) The OspF family of type III effectors, conserved inShigella,Salmonellaand the plant pathogenP. syringae, harbors a novel phosphothreonine lyase activity that specifically and irreversibly “dephosphorylates” host MAPKs, leading to kinase inactivation and inhibited cytokine production. 2) TheLegionellatype IV effector LegK1 mimics host IKK to phosphorylate IkBa, which results in ubiquitination and degradation of IkBaand consequent activation of host anti-apoptotic NF-kB signaling. 3) Type III effectors CHBP (Burkholderia) and Cif (EPEC) use a papain-like catalytic activity to deamidate ubiquitin and ubiquitin-like protein NEDD8. This leads to dysfunctioning of host ubiquitin-proteasome system, and therefore many important cellular processes such as cell cycle progression become abnormal. We believe that such kind of research and discovery is not only revealing in bacterial pathogenesis, but also provides an unprecedented unique angle for studying the mechanism of eukaryotic signal transduction. Meanwhile, we are also interested in how the host uses its innate immunity system to counteract bacterial infection, particularly the inflammasome pathway in macrophages. Macrophage senses many kinds of pathogen-derived molecular patterns and thereby activates the cytoplasmic inflammasome complex, and this leads to Il-1bproduction and inflammatory cell death of the macrophage. The NOD-like receptor in inflammasome is required for sensing bacteria-derived signals. However, little is known about how the inflammasome is assembled/activated, and the signaling cascade upstream of the inflammasome remains obscure. We are combining multiple approaches including biochemical reconstitution, cell biology and mouse genetics to identify new components in pathogen-induced inflammasome activation and to further reveal the underlying biochemical mechanism.

1. Zhou Z, He H, Wang K, Shi X, Wang Y, Su Y, Wang Y, Li D, Liu W, Zhang Y, Shen L, Han W, Shen L, Ding J,Shao F.(2020) Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells.Science, 368, 6494.

2. Wang Q, Wang Y, Ding J, Wang C, Zhou X, Gao W, Huang H,Shao F, Liu Z.(2020) A bioorthogonal system reveals antitumour immune function of pyroptosis.Nature, 579,421-426.

3. Wang K, Sun Q, Zhong X, Zeng M, Zeng H, Shi X, Li Z, Wang Y, Zhao Q,Shao F, Ding J.(2020) Structural mechanism for GSDMD targeting by autoprocessed caspases in pyroptosis.Cell, 2020,180, 941-955.

4. Xu Y, Zhou P, Cheng S, Lu Q, Nowak K, Hopp A, Li L, Shi X, Zhou Z, Gao W, Li D, He H, Liu X, Ding J, Hottiger M,Shao F. (2019) A Bacterial effector reveals the V-ATPase-ATG16L1 axis that initiates xenophagy,Cell, 178, 1-15.

5. Zhou P, She Y, Dong N, Li P, He H, Borio A, Wu Q, Lu S, Ding X, Cao Y, Xu Y, Gao W, Dong M, Ding J, Wang DC, Zamyatina A,Shao F.(2018) Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose,Nature, 561, 122-126 (Published online Aug 15).

6. Li P, Jiang W, Yu Q, Liu W, Zhou P, Li J, Xu J, Xu B, Wang F &Shao F.(2017) Ubiquitination and degradation of GBPs by aShigellaeffector to suppress host defense,Nature, 551, 378-383 (Published online Oct 11).

7. Wang Y, Gao W, Shi X, Ding J, Liu W, He H, Wang K &Shao F. (2017) Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a Gasdermin,Nature, 547 (7661), 99-103 (Published online May 01).

8. Ding J, Wang K, Liu W, She Y, Sun Q, Shi J, Sun H, Wang DC &Shao F. (2016) Pore-forming activity and structural autoinhibition of the Gasdermin family,Nature(Article), 535 (7610), 111-116 (Published online 08 June 2016).

9. Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, Zhuang Y, Cai T, Wang F &Shao F. (2015) Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death,Nature(Article), 526, 660–665.

10. Lu Q, Yao Q, Xu Y, Li L, Li S, Liu Y, Gao W, Niu M, Sharon M, Ben-Nissan G, Zamyatina A, Liu X, Chen S &Shao F. (2014) An iron-containing dodecameric heptosyltransferase family modifies bacterial autotransporters in pathogenesis,Cell Host & Microbe, 16, 351–363.

11. Shi J, Zhao Y, Wang Y, Gao W, Ding J, Li P, Hu L &Shao F. (2014) Inflammatory caspases are innate immune receptors for intracellular LPS,Nature(Article), 514, 187-192 (Published online 06 August 2014).

12. Xu H, Yang J, Gao W, Li L, Li P, Zhang L, Gong YN, Peng X, Xi JJ, Chen S, Wang F,Shao F. (2014) Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome.Nature, 513, 237–241 (Published online 11 June 2014).

13. Li S, Zhang L, Yao Q, Li L, Dong N, Rong J, Gao W, Ding X, Sun L, Chen X, Chen S &Shao F. (2013) Pathogen blocks host death receptor signaling by arginine GlcNAcylation of death domains.Nature, 501, 242-246.

14. Yang J, Zhao Y, Shi J &Shao F. (2013) Human NAIP and mouse NAIP1 recognize bacterial type III secretion needle protein for inflammasome activation.Proc. Natl. Acad. Sci., 110, 14408-13.

15. Dong N, Zhu Y, Lu Q, Hu L, Zheng Y,Shao F. (2012) Structurally distinct bacterial TBC-like GAPs link Arf GTPase to Rab1 inactivation to counteract host defenses.Cell, 150, 1029-41.

16. Zhang L, Ding X, Cui J, Xu H, Chen J, Gong YN, Hu L, Zhou Y, Ge J, Lu Q, Liu L, Chen S,Shao F. (2012) Cysteine methylation disrupts ubiquitin-chain sensing in NF-kB activation.Nature, 481, 204-8.

17. Zhao Y, Yang J, Shi J, Gong YN, Lu Q, Xu H, Liu L,Shao F. (2011) The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus.Nature, 477, 596–600.

18. Cui J, Yao Q, Li S, Ding X, Lu Q, Mao H, Liu L, Zheng N, Chen S,Shao F. (2010) Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family.Science, 329, 1215-8.

19. Li H, Xu H, Zhou Y, Zhang J, Long C, Li S, Chen S, Zhou JM,Shao F. (2007) The phosphothreonine lyase activity of a bacterial type III effector family.Science, 315, 1000-3.

Research articles:

1. Zhang C, Song J, Huang H, Fan X, Huang L, Deng J, Tu B, Wang K, Li J, Zhou M, Yang C, Zhao Q, Yang T, Wang L, Zhang J, Xu R, Jiao Y, Shi M,Shao F, Sékaly R, Wang F (2020)NLRP3 inflammasome induces CD4+ T cell loss in chronically HIV-1–infected patients,The Journal of Clinical Investigation, 131 (6).

2. Karmakar M, Minns M, Greenberg E, Diaz-Aponte J, Pestonjamasp K, Johnson J, Rathkey J, Abbott D, Wang K,Shao F, Catz S, Dubyak G, Pearlman E (2020)N-GSDMD trafficking to neutrophil organelles facilitates IL-1β release independently of plasma membrane pores and pyroptosis,Nature communications, 11, 1-14.

3. Xu H, Shi J, Gao H, Liu Y, Yang Z,Shao F, Dong N. (2019)The N‐end rule ubiquitin ligase UBR2 mediates NLRP1B inflammasome activation by anthrax lethal toxin,The EMBO journal, 38, e101996.

4. Ji C, Du S, Li P, Zhu Q, Yang X, Long C, Yu J,Shao F, Xiao J. (2019)Structural mechanism for guanylate-binding proteins (GBPs) targeting by the Shigella E3 ligase IpaH9. 8,PLoS pathogens, 15, e1007876.

5. Wu C, Lu W, Zhang Y, Zhang G, Shi X, Hisada Y, Grover S, Zhang X, Li L, Xiang B, Shi J, Li X, Daugherty A, Smyth S, Kirchhofer D, Shiroishi T,Shao F, Mackman N, Wei Y, Li Z. (2019)Inflammasome activation triggers blood clotting and host death through pyroptosis,Immunity, 50, 1401-1411.

6. Ding J, Pan X, Du L, Yao Q, Xue J, Yao H, Wang D, Li S,Shao F. (2019) Structural and functional insights into host death domains inactivation by the bacterial arginine GlcNAcyltransferase effector,Molecular Cell, 74, 1-14.

7. Rathinam V, Zhao Y,Shao F. (2019) Innate immunity to intracellular LPS,Nature Immunology, 20, 527-533.

8. Xu H, Shi J, Gao H, Liu Y, Yang Z,Shao F, Dong N. (2019) The N-end rule ubiquitin ligase UBR2 mediates NLRP1B inflammasome activation by anthrax lethal toxin,EMBO, 38(13):e101996.

9. Liu W, Zhou Y, Peng T, Zhou P, Ding X, Li Z, Chen S, Hang HC, andShao F. (2018) N^e-fatty acylation of multiple membrane-associated proteins byShigellaIcsB effector to modulate host function,Nature Microbiology, 3, 996-1009.

10. Adanitsch F, Shi J,Shao F, Beyaert R, Heine H, and Zamyatina A. (2018) Synthetic glycan-based TLR4 agonists targeting Caspase-4/11 for the development of adjuvants and immunotherapeutics,Chem. Sci., 9, 3957-3963.

11. Yang J, Zhao Y, Li P, Yang Y, Zhang E, Zhong M, Li Y, Zhou D, Cao Y, Lu M,Shao F#, Yan H#. (2018) Sequence determinants of specific pattern-recognition of bacterial ligands by the NAIP-NLRC4 inflammasome,Cell Discovery,4: 22. doi: 10.1038/s41421-018-0018-1 (# corresponding authors).

12. Lin R, Feng Q, Li P, Zhou P, Wang R, Liu Z, Wang Z, Qi X, Tang N,Shao F, Luo M. (2018). A hybridization-chain-reaction-based method for amplifying immunosignals,NatureMethods, 15, 275-278.

13. Xu B, Jiang M, Chu Y, Wang W, Chen D, Li X, Zhang Z, Zhang D, Fan D, Nie Y,Shao F, Wu K, Liang J. (2018) Gasdermin D plays a key role as a pyroptosis executor of non-alcoholic steatohepatitis in humans and mice,J Hepatol.,68, 773-782.

14. Zhou Y, Huang C, Yin L, Wan M, Wang X, Li L, Liu Y, Wang Z, Fu P, Zhang N, Chen S, Liu X,Shao F, Zhu Y. (2017). N^ε-Fatty acylation of Rho GTPases by a MARTX toxin effector,Science. 358, 528-531.

15. Sheng X, You Q, Zhu H, Chang Z, Li Q, Wang H, Wang C, Wang H, Hui L, Du C, Xie X, Zeng R, Lin A, Shi D, Ruan K, Yan J, Gao GF,Shao F, Hu R. (2017) Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK,PLoS Pathogen,13(7): e1006534.

16. Litvak Y, Sharon S, Hyams M, Zhang L, Kobi S, Katsowich N, Dishon S, Nussbaum G, Dong N,Shao F, and Rosenshine I. (2017) Epithelial cells detect functional type III secretion system of enteropathogenicEscherichia colithrough a novel NF-κB signaling pathway.PLoS Pathogen, 13(7): e1006472.

17. Dong N, Niu M, Hu L, Yao Q, Zhou R, andShao F. (2016) Modulation of membrane phosphoinositide dynamics by the phosphatidylinositide 4-kinase activity of theLegionellaLepB effector,Nature Microbiology, 2:16236.

18. Thurston TL, Matthews SA, Jennings E, Alix E,Shao F, Shenoy AR, Birrell MA, Holden DW. (2016) Growth inhibition of cytosolicSalmonellaby caspase-1 and caspase-11 precedes host cell death,Nature Communications, 7:13292.

19. Gao W, Yang J, Liu W, Wang Y,Shao F. (2016) Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation,Proc. Natl. Acad. Sci., 113, E4857-66.

20. Zhang Q, Zhou A, Li S, Ni J, Tao J, Lu J, Wan B, Li S, Zhang J, Zhao S, Zhao GP,Shao F, Yao YF (2016) Reversible lysine acetylation is involved in DNA replication initiation by regulating activities of initiator DnaA inEscherichia coli,Sci Rep., 6: 30837.

21. Aubert DF, Xu H, Yang J, Gao W, Li L, Chen S, Valvano MA,Shao F. (2016) ABurkholderiatype VI effector deamidates Rho GTPases to activate the Pyrin inflammasome,Cell Host & Microbe, 19, 664-674.

22. Zhao Y, Shi J, Shi X, Wang Y, Wang F &Shao F. (2016) Genetic functions of the NAIP family of inflammasome receptors for bacterial ligands in mice,Journal of Experimental Medicine, 213(5), 647-56.

23. Zanoni I, Tan Y, Di Gioia M, Broggi A, Ruan J, Shi J, Donado CA,Shao F, Wu H, Springstead JR, Kagan JC. (2016) An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells,Science, 352(6290), 1232-6.

24. Hu Z, Zhou Q, Zhang C, Fan S, Cheng W, Zhao Y,Shao F, Wang HW, Sui SF, Chai J. (2015) Structural and biochemical basis for induced self-propagation of NLRC4,Science, 350, 399-404.

25. Lu Q, Xu Y, Yao Q, Niu M andShao F. (2015) A polar-localized iron-binding protein determines the polar targeting ofBurkholderiaBimA autotransporter and actin tail formation,Cellular Microbiology, 17, 408–424.

26. Yao Q, Zhang L, Wan X, Chen J, Hu L, Ding X, Li L, Karar J, Peng H, Chen S, Huang N, Rauscher FJ,Shao F. (2014) Structure and Specificity of the Bacterial Cysteine Methyltransferase Effector NleE Suggests a Novel Substrate in Human DNA Repair Pathway.PLoS Pathogens, 10: e1004522.

27. Yao Q, Lu Q, Wan X, Song F, Xu Y, Zamyatina A, Huang N, Zhu P &Shao F. (2014) A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family,eLife, 3: e03714.

28. Pan M, Li S, Li X,Shao F, Liu L and Hu HG (2014) Synthesis and Specific Antibody Generation of Glycopeptides with Arginine N-GlcNAcylation.Angew Chem Int Ed Engl.,53, 14517-14521 (co-corresponding author).

29. Ding J, Luo AF, Hu L, Wang DC,Shao F. (2014) Structural basis of the ultrasensitive calcium indicator GCaMP6.SCIENCE CHINA Life Sciences,57, 269–274.

30. Li T, Lu Q, Wang G, Xu H, Huang H, Cai T, Kan B, Ge J &Shao F. (2013) SET-domain bacterial effectors target heterochromatin protein 1 to activate host rDNA transcription.EMBO Reports, 14, 733-40.

31. Yu Q, Hu L, Yao Q, Zhu Y, Dong N, Wang DC,Shao F. (2013) Structural analyses of aLegionellaRabGAP effector reveal a new GAP fold that catalytically mimics eukaryotic RasGAP.Cell Research, 23, 775-87.

32. Zhou Y, Dong N, Hu L,Shao F. (2013) TheShigellaType Three Secretion System Effector OspG Directly and Specifically Binds to Host Ubiquitin for Activation.PLoS One, 8: e57558.

33. Yao Q, Cui J, Wang J, Li T, Wan X, Luo T, Gong YN, Xu Y, Huang N,Shao F. (2012) Structural mechanism of ubiquitin and NEDD8 deamidation catalyzed by bacterial effectors that induce macrophage-specific apoptosis.Proc. Natl. Acad. Sci., 109, 20395-400.

34. Ku B, Lee KH, Park WS, Yang CS, Ge J, Lee SG, Cha SS,Shao F, Heo WD, Jung JU, Oh BH. (2012) VipD ofLegionella pneumophilatargets activated Rab5 and Rab22 to interfere with endosomal trafficking in macrophages.PLoS Pathogen, 8: e1003082.

35. Ge J, Gong YN, Xu Y,Shao F. (2012) Preventing bacterial DNA release and absent in melanoma 2 inflammasome activation by aLegionellaeffector functioning in membrane trafficking.Proc. Natl. Acad. Sci., 109, 6193-8.

36. He S, Liang Y,Shao F, Wang X. (2011) Toll-like receptors activate programmed necrosis in macrophages through a receptor-interacting kinase-3–mediated pathway.Proc. Natl. Acad. Sci., 108, 20054-9.

37. Li C, Tu S, Wen S, Li S, Chang J,Shao F, and Lei X. (2011) Total Synthesis of the G2/M DNA Damage Checkpoint Inhibitor Psilostachyin C.The Journal of Organic Chemistry, 76, 3566-70.

38. Gong YN, Wang X, Wang J, Yang Z, Li S, Yang J, Liu L, Lei X,Shao F. (2010) Chemical probing reveals insights into the signaling mechanism of inflammasome activation.Cell Research, 20, 1289-305.

39. Dong N, Liu L,Shao F. (2010) A bacterial effector targets host DH-PH domain RhoGEFs and antagonizes macrophage phagocytosis.The EMBO J., 29, 1363-76.

40. Zhu Y, Hu L, Zhou Y, Yao Q, Liu L,Shao F. (2010) Structural mechanism of host Rab1 activation by the bifunctionalLegionellatype IV effector SidM/DrrA.Proc. Natl. Acad. Sci., 107, 4699-704.

41. Ge J, Xu H, Li T, Zhou Y, Zhang Z, Li S, Liu L,Shao F.(2009)ALegionellatype IV effector activates the NF-κB pathway by phosphorylating the IκB family of inhibitors.Proc. Natl. Acad. Sci., 106, 13725-30.

42. Chen Y, Yang Z, Meng M, Zhao Y, Dong N, Yan H, Liu L, Ding M, Peng, HB,Shao F. (2009) Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement.Mol. Cell,35, 841-55.

43. Dowen RH, Engel JL,Shao F, Ecker JR, Dixon JE. (2009) A family of bacterial cysteine protease type III effectors utilizes acylation-dependent and -independent strategies to localize to plasma membranes.J. Biol. Chem., 284, 15867-79.

44. Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao, R, Liu X, Huang N, Chen S, Liu L,Shao F. (2009) A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.Proc. Natl. Acad. Sci., 106, 3716-21.

45. Zhu Y., Li H., Hu L., Wang, J., Zhou Y., Pang Z., Liu L.,Shao F. (2008) Structure of aShigellaeffector reveals a new class of ubiquitin ligases.Nature Structural & Molecular Biology, 15, 1302-8.

46. Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC,Shao F. (2007) Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase.Mol. Cell, 28, 899-913.

47. Zhang J,Shao F, Li Y, Cui H, Chen L, Li H, Zou Y, Long C , Lan L, Chai J, Chen S, Tang X, Zhou JM. (2007) APseudomonas syringaeeffector inactivates MAPKs to suppress PAMP-induced immunity.Cell Host & Microbe, 1, 175-85.

48. Alto NM,Shao F, Lazar CS, Brost RL, Chua G, Mattoo, SM, McMahon SA, Ghosh P, Hughes TR, Boone C, Dixon JE. (2006) Identification of a bacterial type III effector family with G-protein mimicry functions.Cell,124, 133-45.

49. Armstrong MB, Bian X, Liu Y, Subramanian C, Ratanaproeska AB,Shao F, Yu V, Kwok R, Opipari AW, Jr., Castle VP. (2006) Signaling from p53 to NF-kappaB determines chemotherapy responsiveness of neuroblastoma.Neoplasia, 8, 964-74.

50. Zhu M,Shao F, Innes RW, Dixon JE, Xu Z. (2004) The crystal structure ofPseudomonasavirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site.Proc. Natl. Acad. Sci., 101, 302-7.

51.Shao F, Golstein, C, Ade J, Stoutemyer M., Dixon JE, Innes RW. (2003) Cleavage ofArabidopsisPBS1 by a bacterial type III effector.Science, 301, 1230-3.

52.Shao F, Vacratsis PO, Bao Z, Bowers KE, Fierke CA, Dixon JE. (2003) Biochemical characterization of theYersiniaYopT protease: cleavage site and recognition elements in Rho GTPases.Proc. Natl. Acad. Sci., 100, 904-9.

53.Shao F, Merritt PM, Bao Z, Innes RW, Dixon JE. (2002) AYersiniaeffector and aPseudomonasavirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis.Cell, 109, 575-88.

54. Bian X, McAllister-Lucas LM,Shao F, Schumacher KR, Feng Z, Porter AG, Castle VP, Opipari AW, Jr. (2001) NF-kappa B activation mediates doxorubicin-induced cell death in N-type neuroblastoma cells.J. Biol. Chem.,276, 48921-9.

55.Shao F, Bader MW, Jakob U, Bardwell JC. (2000) DsbG, a protein disulfide isomerase with chaperone activity.J. Biol. Chem., 275, 13349-52.

56. Wang CG, He XL,Shao F, Liu W, Ling MH, Wang DC, Chi CW. (2001) Molecular characterization of an anti-epilepsy peptide from the scorpionButhus martensi Karsch.Eur. J. Biochem.,268, 2480-5.

57.Shao F, Hu Z, Xiong YM, Huang QZ, Wang CG, Zhu RH, Wang DC. (1999) A new antifungal peptide from the seeds ofPhytolacca americana: characterization, amino acid sequence and cDNA cloning.Biochim. Biophys. Acta, 1430, 262-8.

58.Shao F, Xiong YM, Zhu RH, Ling M, Chi CW, Wang DC. (1999) Expression and purification of the BmK M1 neurotoxin from the scorpionButhus martensii Karsch.Protein Expr. Purif., 17, 358-65.

a)Invited review articles:

59. Schubert KA, Xu Y,Shao F, Auerbuch V. (2020)TheYersiniaType III Secretion System as a Tool for Studying Cytosolic Innate Immune Surveillance,Annual Review of Microbiology, 74, 221-245.

60. Broz P, Pelegrín P,Shao F(2020)The gasdermins, a protein family executing cell death and inflammation,Nature Reviews Immunology, 20,143-157

61. Ding J,Shao F. (2018) Growing a gasdermin pore in membranes of pyroptotic cells.The EMBO J., 37(15). pii: e100067.

62. Dong N,Shao F.(2019)Molecular mechanism and immunological function of pyroptosis,SCIENTIA SINICA, 49, 1606-1634

63. Basler M,Shao F. (2018) Bacterial infection and symbiosis.Mol Biol Cell.29 (6), 683-684.

64. Galluzzi L, ¼,Shao F, ¼, Kroemer, G. (2018) Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.Cell Death & Differentiation, 25 (3), 486-541.

65. Schroder K, Kanneganti TD,Shao F, Broz P. (2018) Mechanisms of Inflammasome Activation.J Mol Biol., 430 (2), 131-132.

66. Ding J,Shao F. (2017) SnapShot: The Noncanonical Inflammasome,Cell, 168:544-544.e1.

67. Shi J, Gao W,Shao F. (2017) Pyroptosis: Gasdermin-mediated programmed necrotic cell death,Trends in Biochemical Sciences, 42 (4), 245–254.

68. Zhao Y,Shao F. (2016) Diverse mechanisms for inflammasome sensing of cytosolic bacteria and bacterial virulence,Current Opinion in Microbiology, 29, 37-42.

69. Lu Q, Li Shan,Shao F. (2015) Sweet talk: protein glycosylation in bacterial interaction with the host.Trends in Microbiology, 23, 630-41 (Cover article).

70. Zhao Y,Shao F. (2015) The NAIP-NLRC4 inflammasome in innate immune detection of bacterial flagellin and type III secretion apparatus.Immunological Reviews, 265, 85-102.

71. Yang J, Zhao Y,Shao F. (2015) Non-canonical activation of inflammatory caspases by cytosolic LPS in innate immunity.Current Opinion in Immunology, 32, 78–83.

72. Yang J, Xu H,Shao F. (2014) The immunological function of familial Mediterranean fever disease protein Pyrin.SCIENCE CHINA Life Sciences, 57, 1156-1161.

73. Zhao Y,Shao F. (2012) NLRC5: a NOD-like receptor protein with many faces in immune regulation.Cell Research, 22, 1099-101.

74. Gong YN,Shao F. (2012) Sensing bacterial infections by NAIP receptors in NLRC4 inflammasome activation.Protein & Cell, 3, 98-105.

75. Ge J,Shao F. (2011) Manipulation of host vesicular trafficking and innate immune defense by Legionella Dot/Icm effectors.Cell. Microbiol., 13, 1870-80.

76. Cui J,Shao F.(2011) Biochemistry and cell signaling taught by bacterial effectors.Trends in Biochemical Sciences, 36, 532-40.

77.Shao F. (2008) Biochemical functions ofYersiniatype III effectors.Current Opinion in Microbiology,11, 21-9.

78. Juris SJ,Shao F, Dixon JE. (2002)Yersiniaeffectors target mammalian signaling pathways.Cell. Microbiol.,4, 201-11 (Co-first author).