Zhaodi Jiang, Ph.D.

Director of Electron microscopy center, TIMBR/NIBS, Beijing, China

Room Number： B416

2010-2017    Ph. D. in Biology, School of Life Sciences, Tsinghua University, China

2006-2010    B. S. in Biotechnology, College of Life Sciences, Jilin University, China

2017-    Director of Electron microscopy center, TIMBR/NIBS, Beijing, China

The electron microscopy center of National Institute of Biological Sciences, Beijing provides advanced services in electron microscopy, from sample preparation to image analysis, for structural investigation of a wide variety of biological samples. We are also dedicated to developing novel genetically encoded tags for EM visualization, and introducing new techniques to expand the application of electron microscopy in biology.

The center is equipped with Thermo Fisher Tecnai spirit G2 (120kV), Wolhwend HPF compact01, Leica EMPACT2, Leica AFS2, Leica UC7/FC7, FEI Vitrobot IV, and we are providing the following services:

1. Sample preparation

Chemical fixation, high pressure freezing, freeze-substitution, cryo-electron microscopy of vitreous sections (CEMOVIS), immunoelectron microscopy (pre-embedding, post-embedding, Tokuyasu cryosectioning technique), negative staining, plunge freezing, correlated light and electron microscopy (CLEM), and so on

2. Electron microscopy analysis

2D imaging, electron tomography, 3D reconstruction of tomograms or serial sections, segmentation and surface rendering, correlated light and electron microscopy

3. User training and consulting

1. Zhao, D., Zou, C.X., Liu, X.M.,Jiang, Z.D., Yu, Z.Q., Suo, F., Du, T.Y., Dong, M.Q., He, W., and Du, L.L. (2020). A UPR-Induced Soluble ER-Phagy Receptor Acts with VAPs to Confer ER Stress Resistance. Mol Cell.

2. Zhang, H., Li, Y., Cui, K., Chen, X., Shang, C., Min, W., Jin, P.,Jiang, Z., Shi, D., Liu, Q., et al.(2020). Male fertility in Mus musculus requires the activity of TRYX5 in sperm migration into the oviduct. J Cell Physiol235, 6058-6072.

3. Yu, Z.Q., Sun, L.L.,Jiang, Z.D., Liu, X.M., Zhao, D., Wang, H.T., He, W.Z., Dong, M.Q., and Du, L.L. (2020). Atg38-Atg8 interaction in fission yeast establishes a positive feedback loop to promote autophagy. Autophagy16, 2036-2051.

4.Jiang, Z., Jin, X., Li, Y., Liu, S., Liu, X.M., Wang, Y.Y., Zhao, P., Cai, X., Liu, Y., Tang, Y., et al.(2020). Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells. Nature methods17, 937-946. co-fist author

5. Zhang, S., Su, Y., Ying, Z., Guo, D., Pan, C., Guo, J., Zou, Z., Wang, L., Zhang, Z.,Jiang, Z., et al.(2019). RIP1 kinase inhibitor halts the progression of an immune-induced demyelination disease at the stage of monocyte elevation. Proc Natl Acad Sci U S A116, 5675-5680.

6. Li, D., Chen, J., Ai, Y., Gu, X., Li, L., Che, D.,Jiang, Z., Li, L., Chen, S., Huang, H., et al.(2019). Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. Molecular Cell.

7. Ying, Z., Pan, C., Shao, T., Liu, L., Li, L., Guo, D., Zhang, S., Yuan, T., Cao, R.,Jiang, Z., et al.(2018). Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. Molecular Cell.

8. Hu, W.,Jiang, Z.D., Suo, F., Zheng, J.X., He, W.Z., and Du, L.L. (2017). A large gene family in fission yeast encodes spore killers that subvert Mendel's law. eLife6.