Sanduo Zheng, Ph.D.

Assistant Professor, TIMBR, Assistant Investigator, NIBS, Beijing

Education

2013    Ph.D. in Structural Biology,

National Institute of Biological Science (TIMBR/NIBS), Beijing & Beijing Normal University, Beijing, China.

2008    B.S., Biological Science,

Lanzhou University, Lanzhou, China

Experience

2019-Present    Assistant Professor, TIMBR, Assistant Investigator, NIBS, Beijing

2015-2019    Postdoctoral Research Fellow, Harvard Medical School, Boston, USA

2013-2015    Postdoctoral Research Fellow, National Institute of Biological Science (TIMBR/NIBS), Beijing, China

Research

Membrane proteins play important roles in virtually every aspect of human physiology and are targets of most modern medicinal drugs. Membrane receptors such as G protein-coupled receptors mediate cellular responses to a variety of extracellular signals and membrane transporters can move ions and molecules across the impermeable membrane. My lab aims to understand the molecular mechanism of membrane receptor signaling and membrane transport through biochemical and structural approaches including X-ray crystallography, electron microscopy and NMR, with a long-term goal to develop novel therapeutics targeting membrane proteins.

Publications

1.Jiang W^#, Wang W^#, Kong Y,Zheng S*(2023). Structural basis for the ubiquitination of G proteinβγsubunits by KCTD5/Cullin3 E3 ligase.Science Advances.9(1-15).

2. Chen S, Teng X,Zheng S*(2022). Molecular basis for the selective G protein signaling of somatostatin receptors.Nature Chemical Biology19: 133-140.

3. Jiang W,ZhengS* (2022). Structural insights into the galanin receptors signaling.Proc. Natl. Acad. Sci. U.S.A.119 (21).

4. Teng X^#, Chen S^#, Wang Q, Chen Z, Wang X, Huang N,Zheng S*(2022). Structural insights into G protein activation by D1 dopamine receptor.Science Advances. 8 (23)

5. Teng X, Chen S, Nie Y, XiaoP, Yu X, Shao Z,Zheng S*(2022). Ligand recognition and biased agonism of the D1 dopamine receptor.Nature communications. 13 (1), 1-11.

6. Ma J^#, Su D^#, Sun Y, Huang X, Liang Y, Fang L, Ma Y, Li W, Liang P*,Zheng S* (2021). Cryo-electron microscopy structure of S-trimer, a subunit vaccine candidate for COVID-19.Journal of Virology. 95 (11).

7. Pascolutti R, Erlandson SC, Burri DJ,Zheng S, Kruse AC* (2020). Mapping and engineering the interaction between adiponectin and T-cadherin.J Bio Chem. 295 (9), 2749-2759

8. Sjodt M, Rohs PD, Gilman MS, Erlandson SC,Zheng S,Green AG, Brock KP, Taguchi A, Kahne D, Walker S, Marks D, Rudner DZ, Bernhardt TG, Kruse AC* (2020). Structural coordination of polymerization and crosslinking by a SEDS-bPBP peptidoglycan synthase complex.Nature Microbiology. 5 (6), 813-820

9. Liu H,Zheng S, Hou X, Liu X, Du K, Lv X, Li Y, Yang F, Li W, Sui J* (2020). Novel Abs targeting the N-terminus of fibroblast growth factor 19 inhibit hepatocellular carcinoma growth without bile-acid-related side-effects.Cancer science.111 (5), 1750-1760

10.Zheng S, Abreu N, Levitz J, Kruse AC* (2019). Structural basis for KCTD-mediated rapid desensitization of GABA_Bsignaling.Nature.567: (127-131).

11.Zheng S, Sham LT, Rubino FA, Brock KP, Robins WP, Mekalanos JJ, Marks DS, Bernhardt TG, Kruse AC*. (2018) Structural and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli.Proc. Natl. Acad. Sci. U.S.A.115 (26): 6709-6714

12. Sham LT,Zheng S, Yakhnina AA, Kruse AC, Berhardt TG*. (2018) Loss of specificity variants of WzxC suggest that substrate recognition is coupled with transporter opening in MOP-family flippase.Mol Microbiol.109 (5): 633-641

13. Huang P^#,Zheng S^#,Wierbowski BM, Kim Y, Nedelcu D, Aravena L, Liu J, Kruse AC., Salic A*. (2018) The structural basis of smoothened activation in Hedgehog signaling.Cell174: 1-13

14. MaMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A^*, Kruse AC*. (2018) Yeast surface display platform for rapid discovery of conformationally selective nanobodies.Nature Structural & Molecular Biology. 25: 289-296

15. Zhou D^#, Zhu X^#,Zheng S, Tan D, Dong MQ, Ye K*. (2018) Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.Protein Cell.10 (2): 120-130

16. Sun Q^#, Zhu X^#, Qi J^#, An W^#, Lan P, Tan D, Chen R, Wang B,Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong M, Ye K*. (2017) Molecular architecture of the 90S small subunit pre-ribosome.eLife. 6: e2208

17. Li D, He W, Liu X,Zheng S, Qi Y, Li H, Mao F, Liu J, Sun Y, Pan L, Du K, Ye K, Li W*, Sui J*. (2017) A potent human neutralizing antibody Fc-dependently reduces established HBV infections.eLife.6: e26738

18. Schmidt HR^#,Zheng S^#,Gurpinar E, Koehl A, Manglik A., Kruse AC*. (2016) Crystal structure of human sigma1 receptor.Nature.532: 527-530

19. Shen M^#, Zhang N^#,Zheng S^#,Zhang W, Zhang H, Lu Z, Su Q, Sun Y, Ye K, Li X*. (2016) Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor.Proc. Natl. Acad. Sci. U.S.A. 113 (40)

20.ZhengS^#, Lan P^#, Liu X, Ye K*. (2014) Interaction between Ribosome Assembly Factors Krr1 and Faf1 Is Essential for Formation of Small Ribosomal Subunit in Yeast.J Bio Chem.289: 22692-703

21.ZhengS, Villa R, Wang J, Feng Y, Wang J, Becker PB, and Ye K*. (2014) Structural basis of X chromosome DNA recognition by MSL2 CXC domain during dosage compensation in Drosophila.Genes & Development28: 2652-2662

22.Zheng S^#, Wang J^#, Feng Y^#*, Wang J, Ye K*. (2012) Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases.PloS One.7: e45437

Invited reviews and comments

1.Zheng S, Kruse AC*. (2019) Ion Channels: Solving a specificity mystery.eLife

2. Alon A^#, Schmidt H^#,Zheng S^#,Kruse AC*. (2017) Structure perspectives on Sigma-1 receptor function.Adv Exp Med Biol. 964:5-13